Search results for "genetics [Transcriptome]"
showing 10 items of 3033 documents
EphrinB2 controls vessel pruning through STAT1-JNK3 signalling
2014
Angiogenesis produces primitive vascular networks that need pruning to yield hierarchically organized and functional vessels. Despite the critical importance of vessel pruning to vessel patterning and function, the mechanisms regulating this process are not clear. Here we show that EphrinB2, a well-known player in angiogenesis, is an essential regulator of endothelial cell death and vessel pruning. This regulation depends upon phosphotyrosine-EphrinB2 signalling repressing c-jun N-terminal kinase 3 activity via STAT1. JNK3 activation causes endothelial cell death. In the absence of JNK3, hyaloid vessel physiological pruning is impaired, associated with abnormal persistence of hyaloid vessel…
The Separation of High and Low Molecular Weight RNA by Precipitation with N-Cetyl- N,N,N-trimethyl-ammoniumbromide
1977
Abstract RNA with a sedimentation constant of 28S and 18S can easily be separated from 4S and 5S RNA. The method depends on the different solubulities of nucleic acids in solutions of the cationic detergent N-cetyl-N,N,N-trimethyl-ammoniumbromide at various ionic strengths. The separation can be achieved with high efficiency in one step.
Bio-phenols determination in olive oils: Recent mass spectrometry approaches
2021
Extra virgin olive oil (EVOO) is largely used in Mediterranean diet, and it is also worldwide apprised not only for its organoleptic properties but also for its healthy effects mainly attributed to the presence of several naturally occurring phenolic and polyphenolic compounds (bio-phenols). These compounds are characterized by the presence of multiple phenolic groups in more or less complex structures. Their content is fundamental in defining the healthy qualities of EVOO and consequently the analytical methods for their characterization and quantification are of current interest. Traditionally their determination has been conducted using a colorimetric assay based on the reaction of Folin…
Quantification of ecdysteroids by immunoassay: comparison of enzyme immunoassay and radioimmunoassay.
1995
Abstract The performance of enzyme immunoassay (EIA) and radioimmunoassay (RIA) in the quantitative analysis of ecdysteroids was compared. The EIA was found to be at least equivalent to the RIA with respect to analytical range and sensitivity and to be more comfortable with respect to safety and time saving. When biological samples were analyzed by both assays a good correlation (r = 0.83) was found. Since the EIA has certain advantages over the RIA, we now recommend the use of the former assay for the quantification of ecdysteroids.
Further Flavonoids and Other Phenolics of Thymus webbianus Rouy
1994
Abstract Seven different flavonoids from the aerial parts of Thymus webbianus were isolated. They were identified by UV spectra, TLC and HPLC-DAD as luteolin, apigenin, eryodictiol, naringenin, luteolin-7-O-glucoside, apigenin-7-O-glucoside and apigenin-6,8-di-C-glucoside. Other phenolics characterized by HPLC-DAD analysis were: protocatechuic, chlorogenic, syringic, p-coumaric and 3,5-dicaffeoylquinic acids.
A lymphocyte-glia connection sets the pace for smoldering inflammation
2021
Successful therapeutic options directly targeting disability progression in patients with multiple sclerosis (MS), a chronic inflammatory disorder of the central nervous system, are lacking. Now, a study published in Nature by Absinta and colleagues profiles a lymphocyte-glia connection at the edge of chronic active lesions that continuously drives neurodegenerative pathways.
Spectroscopic and electrochemical study of interactions between DNA and different salts of 1,4-dihydropyridine AV-153
2020
1,4-dihydropyridines (1,4-DHP) possess important biochemical and pharmacological properties, including antimutagenic and DNA-binding activity. The latter activity was first described for water-soluble 1,4-DHP with carboxylic group in position 4, the sodium salt of the 1,4-DHP derivative AV-153 among others. Some data show the modification of physicochemical properties and biological activities of organic compounds by metal ions that form the salts. We demonstrated the different affinity to DNA and DNA-protecting capacity of AV-153 salts, depending on the salt-forming ion (Na, K, Li, Rb, Ca, Mg). This study aimed to use different approaches to collate data on the DNA-binding mode of AV-153-N…
Tight DNA-protein complexes isolated from barley seedlings are rich in potential guanine quadruplex sequences
2020
Background The concept of chromatin domains attached to the nuclear matrix is being revisited, with nucleus described as a set of topologically associating domains. The significance of the tightly bound to DNA proteins (TBP), a protein group that remains attached to DNA after its deproteinization should be also revisited, as the existence of these interactions is in good agreement with the concept of the topologically associating domain. The work aimed to characterize the DNA component of TBP isolated from barley seedlings. Methods The tight DNA-protein complexes from the first leaves, coleoptiles, and roots of barley seedlings were isolated by purification with chromatography on nitrocell…
Liquid Biopsy in Diagnosis and Prognosis of High-Grade Gliomas; State-of-the-Art and Literature Review
2022
Gliomas, particularly high-grade gliomas, represent the most common and aggressive tumors of the CNS and are still burdened by high mortality and a very poor prognosis, regardless of the type of therapy. Their diagnosis and monitoring rely on imaging techniques and direct biopsy of the pathological tissue; however, both procedures have inherent limitations. To address these limitations, liquid biopsies have been proposed in this field. They could represent an innovative tool that could help clinicians in the early diagnosis, monitoring, and prognosis of these tumors. Furthermore, the rapid development of next-generation sequencing (NGS) technologies has led to a significant reduction in seq…
A Note on Locally ??-compact Spaces
1995
: The local version of the concept of ℰτ-compactness (where ℰ is a class of Hausdorff spaces and ℰ is a cardinal) introduced by the first author as a generalization of Her-rlich's concept of ℰ-compactness (and hence, also of Mrowka's E-compactness) is defined and the corresponding theory is initiated. An essential part of the theory is developed under the additional assumption that all spaces from ℰ are absolute extensors for spaces under consideration. The theory contains as a special case the classical theory of local compactness.